Missouri Botanical Garden Open Conference Systems, TDWG 2013 ANNUAL CONFERENCE

Font Size: 
Next generation sequencing requires next generation publishing: The Biodiversity Data Journal published the first Eukaryotic new species with a fully sequenced transcriptome, DNA barcode and microcomputed tomography
Lyubomir Penev, Pavel Stoev, Ana Komericki, Nesrine Akkari, Shalini Li, Xin Zhou, Scott Edmunds, Chris Hunter, Alexander Weigand, David Porco, Marzio Zapparoli, Teodor Georgiev, Daniel Mietchen, Dave Roberts, Vincent Smith

Building: Grand Hotel Mediterraneo
Room: America del Nord (Theatre I)
Date: 2013-10-30 02:45 PM – 03:00 PM
Last modified: 2013-10-05

Abstract


We demonstrate how new species descriptions can be enhanced by applying next-generation sequencing methods, novel computing and imaging technologies. A cave-dwelling centipede of the genus Eupolybothrus (Chilopoda: Lithobiidae), found in a remote karst region in Knin, Croatia, is the first eukaryotic species that in addition to its traditional morphological characteristics, is described with its fully sequenced transcriptome, DNA barcode (i.e., mitochondrial Cytochrome C Oxidase Subunit I gene; COI), and detailed anatomical X-ray by microtomography (micro-CT) scan. This pilot project illustrates a workflow of producing, storing, publishing and disseminating large data sets associated with the description of a new taxon. It was developed in collaboration between several research institutions and driven by Pensoft Publishers, BGI-Shenzhen and GigaScience. The study is published in the novel Biodiversity Data Journal, launched within the EU-funded project ViBRANT (www.vbrant.eu) as the first journal to provide an integrated workflow for article authoring, peer-review and the open access publication of data and text within a single online collaborative platform. The large-scale data handling, management and storage was provided by the GigaScience GigaDB database (http://dx.doi.org/10.5524/100063), with transcriptomic and annotation data made publically available to the most stringent metadata standards in INSDC (GenBank/EMBL/DDBJ) and GigaDB and the relevant datatype specific repositories.