An exploratory field study demands a framework of biodiversity information systems (BIS), which emphasizes interdisciplinary research and infrastructure development activities that thrive on collaborations at national, regional and global efforts for attaining nature conservation of protected areas in Bangladesh. The results are disseminated in the technological arena among biologists, agriculturists, environmentalists, computer scientists, network specialists, software engineers and their relevant institutions. No one can stem the tremendous daily loss of biodiversity without more uniform and effective information systems. Our study investigated current biodiversity information systems and geospatial applications including GIS (geographic information systems) and GPS (Global Positioning System). The study presents unique tools to educate conservation professionals about the national biodiversity strategic action plan centered around the target areas using existing spatial data structure, regional and local GIS data, imagery services, base map web services, literature reviews, observations, interviews, mapping, stakeholders’ analysis, and species richness and reconnaissance findings through webGIS platforms. The study describes the essential information flows needed for comprehensive data exchange, data indexing, web publication, data quality and accuracy of information, and a data security system. This study provides major input for determining an international, interoperable and user-oriented biodiversity information infrastructure. Overall, the study emphasizes the significance of using technological applications in the fields of BIS adoption techniques compared with using geospatial applications for protected area management. Finally, we focus future research trajectories of a new kind of collaborative approach to drive the methodological agenda and recommend how to further incorporate an open source collaborative WebGIS platform as a communication promoter between the in-situ monitoring and subsequent data analysis to support adaptive territorial management and nature conservation systems at Lawachara National Park.